Analysis of the frequency of formation of HIV mutations associated with resistance to antiretroviral drugs
| Autor: | M.G. Liulchuk, A.M. Shcherbinska, V.V. Kirpicheva |
|---|---|
| Jazyk: | English<br />Ukrainian |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Aktualʹnaâ Infektologiâ, Vol 8, Iss 3-4, Pp 49-53 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2312-413X 2312-4148 2312-413x |
| DOI: | 10.22141/2312-413x.8.3-4.2020.212661 |
| Popis: | Background. The large-scale antiretroviral therapy (ART) for patients with human immunodeficiency virus (HIV) infection is carried out in Ukraine. Continuous monitoring of antiretroviral drugs (ARDs) resistance development is needed. Purpose: to analyze the influence of ART duration, the age and gender of patients on the frequency of the formation of HIV mutations associated with resistance to different classes of ARDs. Material and methods. Blood plasma samples of HIV-infected patients with antiretroviral treatment failure were analyzed. Sequencing of the HIV genome was performed using the ViroSeqTM Genotyping System v.2.1 test systems (Celera Diagnostics, USA) according to the manufacturer instructions. Results. Significantly more often (p ≤ 0.05), HIV drug resistance mutations (DRMs) were detected in HIV-infected men (59.7 %) than in women (40.3 %). It was found that in 59.92 % of cases, the causes of virologic treatment failure were mutations of HIV resistance to at least one antiretroviral drug. The most common nucleoside reverse transcriptase inhibitor mutations were G190S (75.8 %), K101E (72.7 %), Y181C (68.1 %), K103N (38.8 %); and the most common non-nucleoside reverse transcriptase inhibitor mutations were M184V (69 %), K65R (61 %) and D67N (20 %). HIV DRMs associated with protease inhibitor were rare. Conclusions. It was shown that the duration of ART and the age of the patients had no significant effect on HIV resistance to ARDs. The genetic barrier of ARDs was crucial. Against the background of taking drugs with a low genetic barrier, the frequency of HIV DRMs was 5.3 %; administration of drugs with a high genetic barrier was accompanied by the formation of HIV DRMs much less often — in 1.1 % of cases. The bio-behavioral features of male patients have been proven to be a factor associated with risk of HIV DRMs. |
| Databáze: | Directory of Open Access Journals |
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