Investigation of the effect of P14 promoter aberrant methylation on the biological function of human lung cancer cells

Autor: Bing‐Yang Jia, Rong‐Hua Yang, Wen‐Jie Jiao, Kai‐Hua Tian
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Thoracic Cancer, Vol 10, Iss 6, Pp 1388-1394 (2019)
Druh dokumentu: article
ISSN: 1759-7714
1759-7706
DOI: 10.1111/1759-7714.13082
Popis: Background This study was conducted to investigate the effect of P14 promoter aberrant methylation on the biological function of human lung adenocarcinoma cells. Methods We used nested methylation‐specific PCR (NMSP) to detect the methylation status of the p14ARF promoter region in SPCA1 and BEAS2B cell lines. The experimental groups were treated with 5‐aza‐2′‐deoxycytidine (5‐Aza). Quantitative real‐time PCR, Western blot, flow cytometry, and Cell Counting Kit 8 were used to detect the expression of p14ARF messenger RNA and protein in each group, apoptosis, and cell proliferation inhibition, respectively. Results NMSP detected that the p14 promoter region of SPCA1 cells has abnormal methylation status. After treatment with 5‐Aza, the expression of p14ARF messenger RNA and protein in SPCA1 cells (P 0.05). Conclusion Abnormal methylation of the p14ARF promoter region plays an important role in the development of lung cancer cells. Our results suggest the use of P14 promoter aberrant methylation as a therapeutic target for drug research or to improve the sensitivity of other drugs.
Databáze: Directory of Open Access Journals
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