| Autor: |
Cheng-Chia Yu, Ni-Yu Su, Chia-Ming Liu, Li-Chiu Yang, Chung-Hung Tsai, Yu-Chao Chang |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Journal of Dental Sciences, Vol 12, Iss 1, Pp 78-82 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
1991-7902 |
| DOI: |
10.1016/j.jds.2016.11.002 |
| Popis: |
Background/purpose: Gingival overgrowth is a common side effect of medication with the immunosuppressant cyclosporine A (CsA). This study proposed to verify whether Nanog, an embryonic stem cell marker, contributes to gingival overgrowth stimulated with CsA in human gingival fibroblasts (HGFs). Materials and methods: The effect of CsA on HGFs was used to elucidate whether Nanog expression could be induced by CsA using quantitative real-time reverse transcription-polymerase chain reaction and Western blotting. Cell growth in CsA-treated HGFs with Nanog lentivirus-mediated short hairpin RNA interference knockdown was evaluated by tetrazolium bromide reduction assay. Results: CsA upregulated Nanog transcript in HGFs in a dose-dependent manner (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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