Dietary Genistein Influences Number of Acetylcholine Receptors in Female Diabetic Jejunum
| Autor: | Sydney Schacht, Faisal Masood, Shawn Catmull, Robert Dolan, RussL Altabtabaee, Wade Grow, Layla Al-Nakkash |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Journal of Diabetes Research, Vol 2017 (2017) |
| Druh dokumentu: | article |
| ISSN: | 2314-6745 2314-6753 |
| DOI: | 10.1155/2017/3568146 |
| Popis: | Background. Intestinal dysfunction in the ob/ob mouse model of diabetes mimics that seen clinically. Methods. We determined the effects of a 4-week genistein diet (600 mg genistein/kg food) on intestinal function (contractility, morphology, AChR, and motility) in female ob/ob and lean mice. Results. Contractility of the jejunum in response to incrementally increasing concentrations of KCl was comparable in ob/ob females and lean controls regardless of a genistein-diet. There were no changes in the wall thickness measured. We assessed the number of clusters of AChR in the jejunum wall; AChR were decreased by 48% in ob/ob mice versus leans, and the genistein diet reversed this. In utilizing a video-imaging system to evaluate gastrointestinal motility, we determined that the distance between consecutive contractile events was significantly increased by 1.87-fold in ob/ob mice versus leans, and the genistein diet was without effect. Conclusions. These data suggest that slowed intestinal transit in the diabetic ob/ob mouse may be due in part to decreased AChR and decreased contraction events occurring per unit time. A genistein diet rescues the number of AChR to levels of leans yet did not change the number of contractile events. Feeding ob/ob mice a genistein-rich diet has potential therapeutic benefits towards improving the debilitating diabetes-related gastrointestinal dysfunction. |
| Databáze: | Directory of Open Access Journals |
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