| Autor: |
Taha Yassine, Malik Mohamed Khalafalla, Mohamed Mamdouh, Zizy I. Elbialy, Abdallah S. Salah, Aly Ahmedou, Ahmed Mamoon, Ahmed M. El-Shehawi, Hien Van Doan, Mahmoud A.O. Dawood |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Aquaculture Reports, Vol 20, Iss , Pp 100729- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2352-5134 |
| DOI: |
10.1016/j.aqrep.2021.100729 |
| Popis: |
The present study evaluated the enhancement of the growth rate, intestinal health, immune-related genes, and resistance against suboptimal water temperature in common carp (2.01 ± 0.02 g) by dietary paraprobiotics. Fish were fed dietary heat killed Lactobacillus plantarum (LP20) at 0 (control), 50, and 100 mg/kg for 2 months. After the feeding trial fish were exposed to suboptimal water temperature averaged 13.22 ± 0.44 °C for 48 h. The final body weight, weight gain, specific growth rate, and feed conversion ratio of fish fed LP20 at 100 mg/kg were significantly enhanced (P < 0.05). The villi length and width were increased in fish fed LP20 at 50 and 100 mg/kg when compared to the control (P < 0.05). The crypt depth was increased by increasing LP20 in a dose dependent manner with the highest value in the group of fish fed 100 mg/kg (P < 0.05). The number of goblet cells showed higher number in fish fed LP20 at 50 and 100 mg/kg than the control group (P < 0.05). The expression of catalase (CAT) gene did not show any changes in transcription before the thermal stress however after thermal stress fish fed 50 mg/kg had upregulated CAT followed by those fed 100 mg/kg. Glutathione peroxidase (GPx) and superoxide dismutase (SOD) gene expressions showed upregulations in fish fed 50 mg/kg before the thermal stress but after the thermal stress fish fed 100 mg/kg had the highest level of mRNA transcriptions for GPx and SOD genes. In the same manner, the expression of interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and interleukin 10 (IL-10) were significantly upregulated in fish fed LP20 at 50 mg/kg before the thermal stress whereas the expression of these genes was upregulated in fish fed 100 mg/kg. In conclusion, using LP20 at 50−100 mg/kg resulted in enhancing the growth rate, intestinal morphometry, immune and antioxidative related genes. Accordingly, common carp showed high resistance against suboptimal water temperature stress. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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