Effects of Detraining Followed by Aerobic Exercise on Cardiac Stem Cells in Aged Male Rats
| Autor: | Arezoo Eskandari |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Journal of Basic Research in Medical Sciences, Vol 10, Iss 4, Pp 9-17 (2023) |
| Druh dokumentu: | article |
| ISSN: | 2383-0506 2383-0972 |
| Popis: | Introduction: The regenerative potential of cardiac stem and progenitor cells is affected by aging and detraining, with the C-Kit cardiac stem cell expressing the Nkx2.5 transcription factor playing a crucial role. Exercise is known to enhance organ regeneration during aging, but the mechanisms involved in new cardiomyocyte formation during physiological cardiac remodeling remain unclear. Material & Methods: Eighteen aged Wistar rats (~440g) were divided into three groups: Control (CO), aerobic training (AT) (5 days per week, 50-75% of maximum speed) for six weeks, and detraining (DT) for four weeks. RT-PCR analysis determined Nkx2.5 gene expression, while immunohistochemical staining identified C-kit-positive and Ki67-positive cardiac progenitor cells. Results: In heart tissue, C-Kit and Ki67 values significantly differed between the control–training (P=0.001) and training-detraining (P=0.001) groups but not between the control and detraining groups for C-Kit (P=0.502) and Ki67 (P=0.475). Nkx2.5 exhibited a significant difference between control-training (P=0.001), training-detraining (P=0.001), and control-detraining (P=0.006). Conclusion: Exercise increased the proliferation of heart stem cells, activating C-Kit differentiation and elevating Nkx2.5 expression, thereby delaying the effects of aging. However, detraining significantly impacted heart stem cell function, emphasizing the importance of sustained exercise for optimal cardiac health. |
| Databáze: | Directory of Open Access Journals |
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