| Autor: |
Xin Huang, Thomas Nero, Ranjuna Weerasekera, Katherine H. Matej, Alex Hinbest, Zhaowei Jiang, Rebecca F. Lee, Longjun Wu, Cecilia Chak, Japinder Nijjer, Isabella Gibaldi, Hang Yang, Nathan Gamble, Wai-Leung Ng, Stacy A. Malaker, Kaelyn Sumigray, Rich Olson, Jing Yan |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2041-1723 |
| DOI: |
10.1038/s41467-023-37660-0 |
| Popis: |
Abstract Bacterial biofilms are formed on environmental surfaces and host tissues, and facilitate host colonization and antibiotic resistance by human pathogens. Bacteria often express multiple adhesive proteins (adhesins), but it is often unclear whether adhesins have specialized or redundant roles. Here, we show how the model biofilm-forming organism Vibrio cholerae uses two adhesins with overlapping but distinct functions to achieve robust adhesion to diverse surfaces. Both biofilm-specific adhesins Bap1 and RbmC function as a “double-sided tape”: they share a β-propeller domain that binds to the biofilm matrix exopolysaccharide, but have distinct environment-facing domains. Bap1 adheres to lipids and abiotic surfaces, while RbmC mainly mediates binding to host surfaces. Furthermore, both adhesins contribute to adhesion in an enteroid monolayer colonization model. We expect that similar modular domains may be utilized by other pathogens, and this line of research can potentially lead to new biofilm-removal strategies and biofilm-inspired adhesives. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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