| Autor: |
Yue Zhou, Yumeng Xue, Xubo Lin, Menglong Duan, Weili Hong, Lina Geng, Jin Zhou, Yubo Fan |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Smart Materials in Medicine, Vol 5, Iss 1, Pp 140-152 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2590-1834 |
| DOI: |
10.1016/j.smaim.2023.10.002 |
| Popis: |
The rapid progress in point-of-care testing (POCT) has become a promising decentralized patient-centered approach for the control of infectious diseases, especially in resource-limited settings. POCT devices should be inexpensive, rapid, simple operation and preferably require no power supply. Here, we developed a simple bacterial sensing platform that can be operated by a smartphone for bacteria identification and antimicrobial susceptibility testing (AST) based on using a polydiacetylene (PDA) arrayed membrane chip. Each PDA array produced a unique color ‘fingerprint’ pattern for each bacteria based on different modes of action of toxins from bacteria on biomimetic lipid bilayers within PDA-lipid assemblies. We show that the PDA-based device can detect viable cells of bacteria as low as 104 CFU/mL within 1.5 h compared with several days of conventional bacterial identification, with the aid of a smartphone app. The device can also be used for an antimicrobial susceptibility test (AST) for at least two broad-spectrum antimicrobials within 4 h and provide identification of antimicrobial susceptibility and resistance, enabling the selection of appropriate therapies. This PDA-based sensing platform provides an alternative way for bacterial detection and could be used as a portable and inexpensive POCT device for the rapid detection of bacterial infection in limited-resource settings. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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