Genotyping of Toxoplasma gondii in domestic animals from Campeche, México, reveals virulent genotypes and a recombinant ROP5 allele

Autor: Claudia Patricia Rico-Torres, Luis Fernando Valenzuela-Moreno, Elvira Robles-González, Alvar Alonzo Cruz-Tamayo, Miguel Huchin-Cab, Jonathan Pérez-Flores, Lizbeth Xicoténcatl-García, Héctor Luna-Pastén, Luz Belinda Ortiz-Alegría, Irma Cañedo-Solares, Carlos Cedillo-Peláez, Fernando García-Lacy, Heriberto Caballero-Ortega
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Parasitology, Vol 151, Pp 363-369 (2024)
Druh dokumentu: article
ISSN: 00311820
0031-1820
1469-8161
DOI: 10.1017/S0031182024000106
Popis: Toxoplasma gondii has at least 318 genotypes distributed worldwide, and tropical regions usually have greater genetic diversity. Campeche is a state located in the southeastern region of México and has favourable climate conditions for the replication and dissemination of this protozoan, similar to those in South American countries where broad genetic diversity has been described. Thus, in this study, 4 T. gondii isolates were obtained from tissues of stray dogs and free-range chickens in Campeche, México, and were genotyped by Mn-PCR-RFLP with 10 typing markers (SAG1, altSAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico) and 5 virulence markers (CS3, ROP16, ROP17, ROP18 and ROP5) to provide new information about the distribution and virulence prediction of T. gondii genotypes. Two isolates of T. gondii genotype #116 and 2 of genotype #38 were obtained from stray dogs and chickens, respectively. The parasite load found in these species was between
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