Genotyping of Toxoplasma gondii in domestic animals from Campeche, México, reveals virulent genotypes and a recombinant ROP5 allele
Autor: | Claudia Patricia Rico-Torres, Luis Fernando Valenzuela-Moreno, Elvira Robles-González, Alvar Alonzo Cruz-Tamayo, Miguel Huchin-Cab, Jonathan Pérez-Flores, Lizbeth Xicoténcatl-García, Héctor Luna-Pastén, Luz Belinda Ortiz-Alegría, Irma Cañedo-Solares, Carlos Cedillo-Peláez, Fernando García-Lacy, Heriberto Caballero-Ortega |
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Jazyk: | angličtina |
Rok vydání: | 2024 |
Předmět: | |
Zdroj: | Parasitology, Vol 151, Pp 363-369 (2024) |
Druh dokumentu: | article |
ISSN: | 00311820 0031-1820 1469-8161 |
DOI: | 10.1017/S0031182024000106 |
Popis: | Toxoplasma gondii has at least 318 genotypes distributed worldwide, and tropical regions usually have greater genetic diversity. Campeche is a state located in the southeastern region of México and has favourable climate conditions for the replication and dissemination of this protozoan, similar to those in South American countries where broad genetic diversity has been described. Thus, in this study, 4 T. gondii isolates were obtained from tissues of stray dogs and free-range chickens in Campeche, México, and were genotyped by Mn-PCR-RFLP with 10 typing markers (SAG1, altSAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico) and 5 virulence markers (CS3, ROP16, ROP17, ROP18 and ROP5) to provide new information about the distribution and virulence prediction of T. gondii genotypes. Two isolates of T. gondii genotype #116 and 2 of genotype #38 were obtained from stray dogs and chickens, respectively. The parasite load found in these species was between |
Databáze: | Directory of Open Access Journals |
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