Relevant biological interactions biomimicked by capillary electromigration techniques

Autor: Katriina Lipponen, Susanne K. Wiedmer, Marja-Liisa Riekkola
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Journal of Chromatography Open, Vol 1, Iss , Pp 100020- (2021)
Druh dokumentu: article
ISSN: 2772-3917
DOI: 10.1016/j.jcoa.2021.100020
Popis: The potential of using capillary electromigration techniques for biomimicking essential biological interactions is described. The most commonly used methodologies with their exploitation in calculations of kinetics and biomolecular interaction data are briefly discussed. Experimental approaches for bimolecular interactions with some selected examples, including lipoproteins, glycosaminoglycan, liposomes and some DNA or RNA interactions, are enlighted including the affinity constants for some selective important interactions. The review demonstrates that capillary electromigration techniques can be used as alternative biomimicking tools for the determination of important biological interactions. This review covers selected papers published in 2003-2021.
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