Investigation of Chemopreventive and Antiproliferative Potential of
| Autor: | Bushra Ahmad PhD, Muhammad Rashid Khan PhD, Naseer Ali Shah PhD, Tamara P. Kondratyuk PhD, Nadeem Ahmed PhD, John M. Pezzuto PhD, Asma Gul PhD, Aishma Khattak PhD |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Integrative Cancer Therapies, Vol 21 (2022) |
| Druh dokumentu: | article |
| ISSN: | 1552-695X 15347354 |
| DOI: | 10.1177/15347354211069934 |
| Popis: | Context: Carcinogenesis causes much human misery. It is a process involving multistage alterations. Medicinal plants are candidates for beneficial anticancer agents. Objectives: Investigation of anticancer proficiencies of the plant Dicliptera roxburghiana . Material and methods: Crude extract and derived fractions were inspected for their inhibitory potential against nuclear factor KB (NFκB), nitric oxide synthase inhibition, aromatase inhibition and induction of quinone reductase 1 (QR 1). Antiproliferative activity was determined by using various cancer cell lines for example hormone responsive breast cancer cell line MCF-7, estrogen receptor negative breast cancer cell line MDA-MB-231, murine hepatoma cells Hepa 1c1c7, human neuroblastoma cells SK-N-SH and neuroblastoma cells MYCN-2. Results: Ethyl acetate and n -butanol fractions of D. roxburghiana were strongly active against NFκB with IC 50 of 16.6 ± 1.3 and 8.4 ± 0.7 µg/ml respectively with 100% survival. Chloroform fraction of the plant exhibited an induction ratio of 2.4 ± 0.09 with CD value of 17.7 µg/ml. Regarding the nitrite assay, the n -hexane fraction exhibited significant inhibition of NO activity with IC 50 of 17.8 ± 1.25 µg/ml. The n -butanol fraction exhibited strong antiproliferative activity against IcIc-7 cell lines with IC 50 values of 13.6 ± 1.91 µg/ml; against MYCN-2 a cytotoxic effect developed with dose dependence, with IC 50 of 12.6 ± 1.24 µg/ml. In antiproliferative activity against SK-N-SH cell lines, chloroform, ethyl acetate and n -butanol fractions were efficiently active with IC 50 values of 11.2 ± 0.84, 14.6 ± 1.71 and 16.3 ± 1.57 respectively. Discussion and Conclusion: It was demonstrated that various fractions of D. roxburghiana displayed appreciable anticancer characteristics and could be a potent source for the development of anticancer leads. |
| Databáze: | Directory of Open Access Journals |
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