| Autor: |
Steven A. Chambers, Mathew Newman, Melissa M. Frangie, Alena V. Savenka, Alexei. G. Basnakian, Mohammad A. Alam |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Royal Society Open Science, Vol 8, Iss 8 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2054-5703 |
| DOI: |
10.1098/rsos.210395 |
| Popis: |
The discovery of chimeric anti-melanoma agents is reported. These molecules are potent growth suppressors of melanoma cells in vitro with growth inhibition of 50% (GI50) values as low as 1.32 µM. Compounds were more toxic to melanoma cells in vitro than commonly used anti-melanoma agent dacarbazine as measured by TUNEL assay. They induced both caspase-independent apoptosis evident by colocalization of TUNEL with endonuclease G (EndoG) and caspase-mediated apoptosis measured by colocalization of TUNEL with caspase-activated DNase (CAD). In addition, compounds 3 and 5 strongly induced oxidative injury to melanoma cells as measured by TUNEL colocalization with heme oxygenase-1 (HO1). Dacarbazine induced only caspase-independent apoptosis, which may explain why it is less cytotoxic to melanoma cells than compounds 3, 4 and 5. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
