| Autor: |
Shuai Wang, Sailan Zhao, Meixia Du, Kaiqing Mo, Shuwei Pang, Xiuping Zou |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Horticulture Advances, Vol 2, Iss 1, Pp 1-17 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2948-1104 |
| DOI: |
10.1007/s44281-024-00053-4 |
| Popis: |
Abstract Conventional breeding techniques have been effectively utilized for the enhancement of citrus varieties. Nonetheless, traits such as an extended juvenile phase, cross- or self-incompatibility, high genetic heterozygosity, and polyembryony have posed significant challenges and limitations to these methods. The clustered regularly interspaced short palindromic repeats (CRISPR) genome editing has progressively emerged as a vital tool for citrus breeding and research. This article reviews the array of CRISPR/Cas genome editing systems, emphasizes recent advancements in citrus genome editing using CRISPR/Cas, and explores the application of this technology to bolster resistance to citrus canker. The review also covers the development of CRISPR/Cas-mediated transformation and regeneration systems for citrus, alongside approaches for generating transgene-free citrus germplasm. Moreover, the regulatory landscape and societal acceptance of CRISPR/Cas genome editing are examined. Lastly, potential applications of genome editing in citrus breeding are proposed, with attention to prospective challenges. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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Full text from SpringerLink