| Autor: |
Jian eZha, Menglong eHu, Minghua eShen, Bingzhi eLi, Jingyu eWang, Yingjin eYuan |
| Jazyk: |
angličtina |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
Frontiers in Microbiology, Vol 3 (2012) |
| Druh dokumentu: |
article |
| ISSN: |
1664-302X |
| DOI: |
10.3389/fmicb.2012.00355 |
| Popis: |
Reducing xylitol formation is an important step in engineering xylose reductase/xylitol dehydrogenase pathway for ethanol production from xylose in Saccharomyces cerevisiae. In this study, to balance the expression of XYL1 and mutant XYL2 encoding xylose reductase and NADP+-preferring xylitol dehydrogenase for reduced xylitol production, we utilized a strategy combining chassis selection and direct fine-tuning of XYL1 and XYL2 expression. A XYL1 gene under control of various promoters of ADH1, truncated ADH1 and PGK1, and a mutated XYL2 with different copy numbers were constructed into different xylose-utilizing modules, which were then expressed in two yeast chassis W303a and L2612. The strategy enabled us to achieve an improved L2612-derived recombinant strain with XYL1 controlled by promoter PGK1 and with two copies of XYL2. The strain exhibited 21.3% lower xylitol yield and 40.0% higher ethanol yield. The results demonstrate the feasibility of the combinatorial strategy for construction of efficient xylose-fermenting Saccharomyces cerevisiae. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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