SARS-CoV-2 quasi-species analysis from patients with persistent nasopharyngeal shedding

Autor: Pierre Dudouet, Philippe Colson, Sarah Aherfi, Anthony Levasseur, Mamadou Beye, Jeremy Delerce, Emilie Burel, Philippe Lavrard, Wahiba Bader, Jean-Christophe Lagier, Pierre-Edouard Fournier, Bernard La Scola, Didier Raoult
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Scientific Reports, Vol 12, Iss 1, Pp 1-13 (2022)
Druh dokumentu: article
ISSN: 2045-2322
DOI: 10.1038/s41598-022-22060-z
Popis: Abstract At the time of a new and unprecedented viral pandemic, many questions are being asked about the genomic evolution of SARS-CoV-2 and the emergence of different variants, leading to therapeutic and immune evasion and survival of this genetically highly labile RNA virus. The nasopharyngeal persistence of infectious virus beyond 17 days proves its constant interaction with the human immune system and increases the intra-individual mutational possibilities. We performed a prospective high-throughput sequencing study (ARTIC Nanopore) of SARS-CoV-2 from so-called "persistent" patients, comparing them with a non-persistent population, and analyzing the quasi-species present in a single sample at time t. Global intra-individual variability in persistent patients was found to be higher than in controls (mean 5.3%, Standard deviation 0.9 versus 4.6% SD 0.3, respectively, p
Databáze: Directory of Open Access Journals
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