Antibacterial efficacy of neem oil fractions on clinical isolates of endometritic cows

Autor: P BARMAN, M C YADAV, H KUMAR, S K MEUR, M RAWAT
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Indian Journal of Animal Sciences, Vol 79, Iss 7 (2024)
Druh dokumentu: article
ISSN: 0367-8318
2394-3327
DOI: 10.56093/ijans.v79i7.76386
Popis: The objective of this study was to assess in vitro antibacterial activity of neem oil (Azadirachta indica) and its fractions and neem seed aqueous extract on microflora of cervica- vaginal mucus (CVM collected from cows with endometritis.  The CVM samples were obtained from 12 cows during 40-50 days postpartum and were subjected to bacterial isolation and identification. Neem oil was fractionated by 2 approaches, viz. by solvent extraction and by column chromatography using a silica gel column. Neem seed aqueous extract was prepared from dried neem seeds. Isolates (20) were identified, comprising mostly facultative anaerobic bacteria. Non-lactose fermenting (NLF) bacteria belonging to Enterobacteriaceae family was the most frequently isolated bacteria (25%) followed by Escheriachia coli (20%), Corynebacterium sp. And Streptococcus sp. (15% each), Extraction of neem oil with organic solvents, viz. methanol and hexane yielded 4 fractions which were termed methanol miscrible (F1), methanol-immiscible emulsion (f2), hexane miscrible (F3) and hexane-immiscible emulsion (F4). Column chromatography yielded 3 fractions, viz. F5 (first fraction), F6(Second fraction) and F7 (third fraction). The F1 (methanolic) fraction showed the highest antibacterial activity with a sensitivity pattern of 95% followed by F2 and F3 (85% each), F4 (65%), F6 and F7(40% each). Moreover, the neem seed aqueous extract did not possess any antibacterial activity.
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