Preparation of Amyloid Fibril Networks

Autor: Mirren Charnley, Jay Gilbert, Owen Jones, Nicholas Reynolds
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Bio-Protocol, Vol 8, Iss 4 (2018)
Druh dokumentu: article
ISSN: 2331-8325
DOI: 10.21769/BioProtoc.2733
Popis: Networks of amyloid nanofibrils fabricated from common globular proteins such as lysozyme and β-lactoglobulin have material properties that mimic the extracellular microenvironment of many cell types. Cells cultured on such amyloid fibril networks show improved attachment, spreading and in the case of mesenchymal stem cells improved differentiation. Here we describe a detailed protocol for fabricating amyloid fibril networks suitable for eukaryotic cell culture applications.
Databáze: Directory of Open Access Journals