| Autor: |
George Sentis, Catherine Loukogiannaki, Nikos Malissovas, Dionysis Nikolopoulos, Theodora Manolakou, Sofia Flouda, Maria Grigoriou, Aggelos Banos, Dimitrios T. Boumpas, Anastasia Filia |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Frontiers in Immunology, Vol 14 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1664-3224 |
| DOI: |
10.3389/fimmu.2023.1203848 |
| Popis: |
ObjectiveA blood-based biomarker is needed to assess lupus nephritis (LN) disease activity, minimizing the need for invasive kidney biopsies. Long non-coding RNAs (lncRNAs) are known to regulate gene expression, appear to be stable in human plasma, and can serve as non-invasive biomarkers.MethodsTranscriptomic data of whole blood samples from 74 LN patients and 20 healthy subjects (HC) were analyzed to identify differentially expressed (DE) lncRNAs associated with quiescent disease and flares. Weighted gene co-expression network analysis (WGCNA) was performed to uncover lncRNAs with a central role (hub lncRNAs) in regulating key biological processes that drive LN disease activity. The association of hub lncRNAs with disease activity was validated using RT-qPCR on an independent cohort of 15 LN patients and 9 HC. cis- and trans-targets of validated lncRNAs were explored in silico to examine potential mechanisms of their action.ResultsThere were 444 DE lncRNAs associated with quiescent disease and 6 DE lncRNAs associated with flares (FDR |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
