| Autor: |
Takafumi Miyamoto, Song-Iee Han, Hitoshi Shimano |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 2, Iss 3, Pp 100721- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2021.100721 |
| Popis: |
Summary: Disruption of mitochondrial morphology occurs during various diseases, but the biological significance is not entirely clear. Here, we describe a detailed step-by-step protocol for a chemically inducible dimerization system-based synthetic protein device, termed inducible counter mitochondrial morphology. This system allows artificial manipulation of mitochondrial morphology on a timescale of minutes in living mammalian cells. We also describe an AI-assisted imaging processing approach.For complete details on the use and execution of this protocol, please refer to Miyamoto et al., 2021. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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