Antioxidant Effects of Eugenol on Oxidative Stress Induced by Hydrogen Peroxide in Islets of Langerhans Isolated from Male Mouse

Autor: Ali Akbar Oroojan, Narges Chenani, Marzieh An’aam
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: International Journal of Hepatology, Vol 2020 (2020)
Druh dokumentu: article
ISSN: 2090-3448
2090-3456
DOI: 10.1155/2020/5890378
Popis: Background. The antioxidant system in islets of Langerhans is weak, which can lead to diabetes. Meanwhile, the main component of cloves that produce antioxidant effects is eugenol. Accordingly, the present study was conducted to investigate the antioxidant effect of eugenol on oxidative stress induced by hydrogen peroxide (H2O2) in islets of Langerhans isolated from the male mice. Materials and Methods. In this experimental study, adult Naval Medical Research Institute (NMRI) mice (20-25 g) were prepared. The collagenase digestion method was used for dissecting the islets of Langerhans. H2O2 50 μM was administered for 30 min to induce oxidative stress, with 50, 100, and 200 μM of eugenol employed for 2 hours before the administration of H2O2. The experimental groups were divided into five groups: (control, H2O2, and H2O2+eugenol 50, 100, and 200 μM). Finally, the islet’s lipid peroxidation and antioxidants levels were measured by the ELISA assay method. Results. Malondialdehyde (MDA), total antioxidant capacity (TAC), superoxide dismutase (SOD), and catalase (CAT) increased in all groups when compared to the control (P
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