| Autor: |
A. A. Petrov, A. V. Kazantsev, K. A. Panferov, A. A. Chislov, E. A. Kovalchuk, D. A. Kutaev, S. V. Borisevich |
| Jazyk: |
ruština |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Вестник войск РХБ защиты, Vol 7, Iss 4, Pp 384-392 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2587-5728 |
| DOI: |
10.35825/2587-5728-2023-7-4-384-392 |
| Popis: |
Catastrophic pandemic of the particularly dangerous coronavirus SARS-CoV-2 in 2020–2022 and the unexpected spread of the monkeypox pathogen from Africa in 2022, demonstrate the need for an adequate response to biological threats that have exotic infections as their source, overcome the interspecies barrier between animals and humans and have high rates of virulence and contagiousness in relation to the latter. The purpose of the article is to create a technology for constructing means for the express indication of new especially dangerous and exotic infections, which makes it possible to quickly develop a gene diagnostic tool, evaluate its characteristics and launch large-scale production. Materials and methods. The authors used technologies for constructing means for express indication of new especially dangerous and exotic infections based on real-time polymerase chain reaction (RT-PCR-RT-Flu/ Coronavirus) methods, suitable for multiplex identification of coronavirus RNA. The discussion of the results. The developed technology for constructing means for express indication of new especially dangerous and exotic infections was successfully tested at the laboratory base of the FSBЕ «48 Central Research Institute» of the Ministry of Defense of the Russian Federation using the example of designing a «Set of reagents for detecting the RNA of coronaviruses SARS-CoV, MERS-CoV, SARS-CoV-2 and virus influenza A by real-time polymerase chain reaction (RT-PCR-RT-Flu/Coronavirus)», suitable for multiplex identification of coronavirus RNA. Conclusion. As a result of the research carried out to evaluate the equipment available at the laboratory base of the FSBЕ «48 Central Research Institute» of the Ministry of Defense of the Russian Federation, the adaptation and implementation of key production processes, the development and production of express-indication reagents, as well as testing the technology for constructing express-indication means for new especially dangerous and exotic infections, using the example of designing a set of RT-PCR-RV-Flu/Coronavirus reagents, a gene diagnostic platform was created for the development of reagents for the express indication of new especially dangerous and exotic infections. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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