A high throughput biochemical fluorometric method for measuring lipid peroxidation in HDL.

Autor: Theodoros Kelesidis, Christian K Roberts, Diana Huynh, Otoniel Martínez-Maza, Judith S Currier, Srinivasa T Reddy, Otto O Yang
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: PLoS ONE, Vol 9, Iss 11, p e111716 (2014)
Druh dokumentu: article
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0111716
Popis: Current cell-based assays for determining the functional properties of high-density lipoproteins (HDL) have limitations. We report here the development of a new, robust fluorometric cell-free biochemical assay that measures HDL lipid peroxidation (HDLox) based on the oxidation of the fluorochrome Amplex Red. HDLox correlated with previously validated cell-based (r = 0.47, p
Databáze: Directory of Open Access Journals