DspA/E, a Type III Effector Essential for Erwinia amylovora Pathogenicity and Growth In Planta, Induces Cell Death in Host Apple and Nonhost Tobacco Plants
| Autor: | Tristan Boureau, Hayat ElMaarouf-Bouteau, Amélie Garnier, Marie-Noëlle Brisset, Claude Perino, Igor Pucheu, Marie-Anne Barny |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: | |
| Zdroj: | Molecular Plant-Microbe Interactions, Vol 19, Iss 1, Pp 16-24 (2006) |
| Druh dokumentu: | article |
| ISSN: | 1943-7706 0894-0282 |
| DOI: | 10.1094/MPMI-19-0016 |
| Popis: | Erwinia amylovora is responsible for fire blight, a necrotic disease of apples and pears. E. amylovora relies on a type III secretion system (TTSS) to induce disease on hosts and hypersensitive response (HR) on nonhost plants. The DspA/E protein is essential for E. amylovora pathogenicity and is secreted via the TTSS in vitro. DspA/E belongs to a type III effector family that is conserved in several phytopathogenic bacteria. In E. amylovora, DspA/E has been implicated in the generation of an oxidative stress during disease and the suppression of callose deposition. We investigated the fate of DspA/E in planta. DspA/E delivered artificially to apple or tobacco cells by agroinfection induced necrotic symptoms, indicating that DspA/E was probably injected via the TTSS. We confirmed that DspA/E acts as a major cell-death inducer during disease and HR, because the dspA/E mutant is severely impaired in its ability to induce electrolyte leakage in apple and tobacco leaves. Expression of the defense marker gene PR1 was delayed when dspA/E was transiently expressed in tobacco, suggesting that DspA/E-mediated necrosis may be associated with an alteration of defense responses. |
| Databáze: | Directory of Open Access Journals |
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