| Autor: |
Thais Preisser Pontelo, Mauricio Machaim Franco, Taynan Stonoga Kawamoto, Felippe Manoel Costa Caixeta, Ligiane de Oliveira Leme, Nayara Ribeiro Kussano, Marcio Gilberto Zangeronimo, Margot Alves Nunes Dode |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
PLoS ONE, Vol 16, Iss 3, p e0247518 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
1932-6203 |
| DOI: |
10.1371/journal.pone.0247518 |
| Popis: |
This study aimed to evaluate the effect of scriptaid during pre-maturation (PIVM) and/or maturation (IVM) on developmental competence of bovine oocytes. Cumulus-oocyte complexes (COCs) were submitted to PIVM for 6 h in the presence or absence of scriptaid. COCs were distributed into five groups: T1-IVM for 22 h, T2-PIVM for 6 h and IVM for 22 h, T3-PIVM with scriptaid for 6 h and IVM for 22 h, T4-PIVM for 6 h and IVM with scriptaid for 22 h, and T5-PIVM with scriptaid for 6 h and IVM with scriptaid for 22 h. Nuclear maturation, gene expression, cumulus cells (CCs) expansion, and embryo development and quality were evaluated. At the end of maturation, all groups presented the majority of oocytes in MII (P>0.05). Only HAT1 gene was differentially expressed (P0.05). In conclusion, presence of scriptaid in PIVM and/or IVM did not improve developmental competence or embryo quality. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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