Development, Characterization, and Validation of a Sensitive Primate-Specific Quantification Assay for Forensic Analysis
| Autor: | John C. Fox, Christopher A. Cave, James W. Schumm |
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| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 34, Iss 2, Pp 314-322 (2003) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/03342rr02 |
| Popis: | Accurate human-specific DNA quantification is essential for forensic casework analysis. In this work, we describe a microplate-based quantification assay that utilizes the PCR amplification of human-specific TH01 primers. This method enables the reliable quantification of human DNA samples from 0.2 to 40 ng, even in mixtures with nonhuman DNA. Analysis of samples can be semi-automated using 96-well microplates and a spreadsheet-based concentration calculator for high-throughput demands. We have used this quantification method with more than 15 000 forensic samples. |
| Databáze: | Directory of Open Access Journals |
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