Overexpression of GhSWEET42, a SWEET-like gene from cotton, enhances the oil content and seed size

Autor: Deying Wang, Mengxue Du, Jingyu Li, Shuwen Yin, Yongwang Sun, Shangjing Guo
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Biotechnology & Biotechnological Equipment, Vol 37, Iss 1 (2023)
Druh dokumentu: article
ISSN: 13102818
1314-3530
1310-2818
DOI: 10.1080/13102818.2023.2266529
Popis: AbstractSWEET (‘sugars will eventually be exported transporters’) family genes reportedly play a critical role in sugar translocation and oil biosynthesis in various plant species. However, their functions in cotton are unknown. The present study demonstrated that while GhSWEET42 was widely expressed in different cotton tissues, it had the highest expression level in the developing ovules. Hence, it performs a vital role in seed development. We constructed GhSWEET42 transgenic Arabidopsis lines to verify the biological function of this gene and found that the oil content and weight of the seeds produced by the overexpression lines were 18–23% and 19–20% higher, respectively than those of the wild-type. Gas chromatography–mass spectrometry (GC–MS) analysis revealed that it was mainly a relative increase in unsaturated fatty acids (FAs) that contributed to the relative increase in oil content in the transgenic seeds. Moreover, the latter exhibited comparative upregulation of certain genes associated with FA and triacylglycerol biosynthesis as well as cell expansion. GhSWEET42 might work synergistically with the aforementioned genes. This finding indicates that GhSWEET42 may be essential in oil biosynthesis and seed development in cotton. The results of the present work may facilitate further explorations into the molecular mechanism of cottonseed oil biosynthesis as well as the cultivation of novel oil-rich cotton varieties.
Databáze: Directory of Open Access Journals
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje