Responses of osteoblasts under varied tensile stress types induced by stretching basement materials

Autor: Xuezheng Geng, Qiao Li, Yan Yao, Lizhen Wang, Yubo Fan
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: International Journal of Smart and Nano Materials, Vol 15, Iss 2, Pp 279-295 (2024)
Druh dokumentu: article
ISSN: 19475411
1947-542X
1947-5411
DOI: 10.1080/19475411.2024.2338737
Popis: Osteoblasts are mechanosensitive cells. Tensile stress with different conditions, including loading time, frequency, magnitude, etc. would cause varied responses in osteoblasts. However, it was not clarified that the effect of the loading types on the osteoblasts. In this study, we focused on the effect of varied tensile stress types on osteoblasts, including isotropic stretch, biaxial stretch, and uniaxial stretch with the negative ratio of transverse strain to axial strain (NR) −1, 0, and 0.2 respectively. Cell proliferation was determined to be most efficient when stimulated by 6% strain at a frequency of 1 Hz and a negative value of 0 for 1 h/day. The varied strain resulted in a thickening of the F-actin cytoskeleton and a thinning of the nucleus. Nuclear flattening caused Yes-associated protein (YAP) to be transported to the nucleus. It was suggested that the influence of loading types on the mechanobiology responses must be noticed. The mechanism of cell mechanical sensitivity under varied loading types was explored, which would provide good suggestions for designing microstructures to control deformation patterns in bone tissue engineering.
Databáze: Directory of Open Access Journals
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