In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil
| Autor: | Ryan M. Williams, Amanda R. Kulick, Srilakshmi Yedlapalli, Louisa Battistella, Cyrus J. Hajiran, Letha J. Sooter |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Journal of Nucleic Acids, Vol 2014 (2014) |
| Druh dokumentu: | article |
| ISSN: | 2090-0201 2090-021X |
| DOI: | 10.1155/2014/102968 |
| Popis: | Bromacil is a widely used herbicide that is known to contaminate environmental systems. Due to the hazards it presents and inefficient detection methods, it is necessary to create a rapid and efficient sensing device. Towards this end, we have utilized a stringent in vitro selection method to identify single-stranded DNA molecular recognition elements (MRE) specific for bromacil. We have identified one MRE with high affinity (Kd=9.6 nM) and specificity for bromacil compared to negative targets of selection and other pesticides. The selected ssDNA MRE will be useful as the sensing element in a field-deployable bromacil detection device. |
| Databáze: | Directory of Open Access Journals |
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