RECOMBINANT LENTIVIRUS-MEDIATED SILENCING OF ADAPTOR PROTEIN RUK/CIN85 EXPRESSION INFLUENCES BIOLOGICAL RESPONSES OF TUMOR CELLS
| Autor: | A. A. Samoylenko, N. V. Byts, G. V. Pasichnyk, N. V. Kozlova, A. V. Bazalii, D. S. Gerashchenko, S. G. Shandrenko, A. V. Vorotnikov, T. Kietzmann, S. V. Komisarenko, L. B. Drobot |
|---|---|
| Jazyk: | English<br />Ukrainian |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Biotechnologia Acta, Vol 6, Iss 4, Pp 182-189 (2013) |
| Druh dokumentu: | article |
| ISSN: | 2410-7751 2410-776X |
| DOI: | 10.15407/biotech6.04.182 |
| Popis: | Ruk/CIN85 is an adaptor protein that plays important roles in the regulation of cellular processes such as cell death, proliferation and motility. It was recently shown that overexpression of Ruk/CIN85 increases the oncogenic potential of human breast adenocarcinoma MCF-7 cells. It was the aim of the present study to investigate whether inhibition of Ruk/CIN85 expression has an effect on the biological properties of the cells. In order to down-regulate Ruk/CIN85 expression of small interfering RNA-based approach was used. For down-regulation of Ruk/CIN85 lentiviral constructs encoding Ruk/CIN85-specific small hairpin RNA sequences were generated. By using the obtained recombinant lentiviruses it was shown that inhibition of Ruk/CIN85 expression influences biological properties (motility, proliferation, ABCG2 expression, and ROS generation) of various tumour cell types such as human breast adenocarcinoma MCF-7, human colorectal adenocarcinoma HT-29, and Lewis mouse lung carcinoma cells. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|