3D tracking of ferrous iron in the epileptic mouse brain and screening of iron homeostasis regulators with a two-photon fluorescent probe

Autor: Chenwen Shao, Zhang-Peng Chen, Chao Yan, Yong Qian
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: STAR Protocols, Vol 3, Iss 3, Pp 101522- (2022)
Druh dokumentu: article
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2022.101522
Popis: Summary: The development of techniques for tracking active ferrous iron (Fe2+) distribution has greatly promoted the biological studies of iron. Here, we present an innovative application of a 3D two-photon fluorescent probe for Fe2+ tracking in the epileptic mouse brain, which has expanded the toolbox of screening for iron homeostasis regulators and contributed to the discovery of new chemical entities for the treatment of epilepsy.For complete details on the use and execution of this protocol, please refer to Shao et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Databáze: Directory of Open Access Journals