| Autor: |
Marta Lomnytska, Rui Pinto, Susanne Becker, Ulla Engström, Sonja Gustafsson, Christina Björklund, Markus Templin, Jan Bergstrand, Lei Xu, Jerker Widengren, Elisabeth Epstein, Bo Franzén, Gert Auer |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Biomarker Research, Vol 6, Iss 1, Pp 1-14 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
2050-7771 |
| DOI: |
10.1186/s40364-018-0118-y |
| Popis: |
Abstract Background Platelets support cancer growth and spread making platelet proteins candidates in the search for biomarkers. Methods Two-dimensional (2D) gel electrophoresis, Partial Least Squares Discriminant Analysis (PLS-DA), Western blot, DigiWest. Results PLS-DA of platelet protein expression in 2D gels suggested differences between the International Federation of Gynaecology and Obstetrics (FIGO) stages III-IV of ovarian cancer, compared to benign adnexal lesions with a sensitivity of 96% and a specificity of 88%. A PLS-DA-based model correctly predicted 7 out of 8 cases of FIGO stages I-II of ovarian cancer after verification by western blot. Receiver-operator curve (ROC) analysis indicated a sensitivity of 83% and specificity of 76% at cut-off >0.5 (area under the curve (AUC) = 0.831, p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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