Autor: |
Jöran Lücke, Tao Zhang, Dimitra E. Zazara, Philipp Seeger, Jacob R. Izbicki, Thilo Hackert, Samuel Huber, Anastasios D. Giannou |
Jazyk: |
angličtina |
Rok vydání: |
2024 |
Předmět: |
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Zdroj: |
STAR Protocols, Vol 5, Iss 1, Pp 102696- (2024) |
Druh dokumentu: |
article |
ISSN: |
2666-1667 |
DOI: |
10.1016/j.xpro.2023.102696 |
Popis: |
Summary: Forced metastasis models, those in which the step of intravasation is bypassed, can be used to investigate the mechanisms underlying metastasis and evaluate potential therapeutic targets. Here, we present a protocol for using three forced models of lung and liver metastasis to generate metastasis within 3–4 weeks in approximately 99% of injected mice. We describe steps for cancer cell preparation, mouse analgesia and anesthesia; injecting through intrasplenic, intraportal, and intravenous techniques; and daily evaluation of metastasis.For complete details on the use and execution of this protocol, please refer to Giannou et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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