Changes in ghrelin mRNA level, plasma growth hormone and growth performance in dietary energy and protein levels of the diet in broiler chickens

Autor: Karim Nobari, Shokoufe Ghazanfari, Mojtaba Tahmoorespur
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Zdroj: Italian Journal of Animal Science, Vol 9, Iss 3, Pp e56-e56 (2010)
Druh dokumentu: article
ISSN: 1594-4077
1828-051X
DOI: 10.4081/ijas.2010.e56
Popis: We studied the effects of different energy and protein contents of the diet on performance, growth hormone concentration and ghrelin gene expression in broiler chicken. Two experiments were conducted to determine whether different dietary energy and protein levels alter growth hormone concentration and ghrelin mRNA abundance in broiler chicks. Body weight and feed intake were recorded weekly. Blood, carcass traits and proventriculus samples were collected at 21, 42 and 56 days of age. Blood samples assayed for growth hormone (GH) concentration by radio immuno assay (RIA) and ghrelin gene expression from proventriculus tissue was measured by Real Time polymerase chain reaction (PCR). Feed intake and body weight gain increased in broilers fed on low-energy diets compared with those fed on high-energy diets at 21 days of age (P
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