A study of the protein-protein interactions in the phycocyanin monomer from Synechocystis sp. PCC 6803 using a bacterial two-hybrid system

Autor: Simi Shao, Longsheng Zhao, Pingyi Li, Hainan Su, Xiulan Chen, Yuzhong Zhang
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Engineering Microbiology, Vol 2, Iss 2, Pp 100019- (2022)
Druh dokumentu: article
ISSN: 2667-3703
DOI: 10.1016/j.engmic.2022.100019
Popis: Investigations into the intramolecular interactions of the native protein in solution are important to understand its structural stability as well as its potential uses in future applications. In this study, we used a bacterial two-hybrid system to investigate the interaction between the phycocyanin α and β subunits that form the phycocyanin monomer. Key amino acid residues responsible for the interaction between the subunits were identified, providing direct experimental evidence for the intramolecular interaction.
Databáze: Directory of Open Access Journals