| Autor: |
Syavash Yazdian Anari, Nafiseh Saminasab, Mina Ramezani, Azadeh Mohammadgholi |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Journal of Advanced Biomedical Sciences, Vol 14, Iss 1, Pp 1-11 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2783-1523 |
| Popis: |
Background & Objectives: Carbon nanotubes (CNTs) serve as molecular carriers for in vivo and in vitro delivery. Initial studies have suggested that nanotubes in drug delivery can enhance the therapeutic response to anti-cancer drugs. The present study intended to investigate the effect of CNTs carrying tamoxifen (TAM-CNTs) on the induction of apoptosis in the MDA-MB-231 cell line. Materials & Methods: The cells were treated with various concentrations of TAM and TAM-CNTs. The IC50 for these compounds was determined using a MTT assay. The cells were then treated with a lower concentration of IC50. The BAX and BCL-2 genes expression were evaluated by Real-Time PCR and Western blot. Flow cytometry was employed for evaluating apoptosis induction by TAM and TAM-CNTs. Results: The IC50 value of TAM and TAM-CNTs in a 48-hour period was 66.19 mg/mL and 36.59 mg/mL, respectively. The results demonstrated that BAX in the cells treated with TAM and TAM-CNTs was upregulated 3.64 and 7.88 times, respectively (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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