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Recently, there has been an increase in the application of lactic acid bacteria (LAB) for seed fermentation because of the improved functional, technological, and nutritional properties of the fermented seeds. The purpose of this study was to evaluate the effects of selected LAB on the biochemical and antimicrobial features of fermented hemp seeds and their water-, salt-, and ethanol-soluble protein fractions. The results showed that hemp seed medium was suitable for Lactiplantibacillus plantarum, Levilactobacillus brevis, and Lactobacillus acidophilus multiplication (with a LAB count > 109 colony-forming units/g). The biochemical and antimicrobial properties of the fermented hemp seeds strictly depended on the LAB strain used for fermentation. The hemp seeds fermented with L. brevis, which was previously isolated from rye sourdough, presented the highest total phenolic content and phytase, amylase, protease, and antioxidant activities. The hemp seeds fermented with L. acidophilus showed the broadest spectrum of antimicrobial activity against foodborne pathogens. The ethanol-soluble protein fractions from the fermented hemp seeds inhibited the growth of Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, Bacillus subtilis, and Bacillus cereus, while the ethanol-soluble protein fraction from unfermented hemp seeds did not have an antimicrobial effect. The results of ABTS·+ radical scavenging and antimicrobial activity assays by agar well diffusion method confirmed that the tested LAB strains for hemp seed fermentation influenced the antioxidant and antimicrobial activities of protein fractions. |
