The Utilization of the SaLux19-Based Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid and Sensitive Identification of Minute Amounts of a Biological Specimen

Autor:	Ema Ruszova, Daniel Vanek, Walter Stühmer, Ziad Khaznadar, Nidhi Subhashini
Jazyk:	angličtina
Rok vydání:	2024
Předmět:	species identification LAMP point-of-care testing SaLux19 Sus scrofa (wild boar/pig) Science
Zdroj:	Life, Vol 14, Iss 5, p 579 (2024)
Druh dokumentu:	article
ISSN:	14050579 2075-1729
DOI:	10.3390/life14050579
Popis:	Our research has developed a highly sensitive and simple assay to detect small amounts of animal and human biological material in less than 40 min. The handheld SaLux19 device developed at the Max Planck Institute of Experimental Medicine in Göttingen, Germany, was used to validate our concept. The proposed system uses isothermal amplification of DNA in a rapid assay format. Our results show that the assay can detect Sus scrofa nucleic acids with very high sensitivity and specificity. This detection system has potential for forensic scenarios.
Databáze:	Directory of Open Access Journals
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