Characteristics of a cytokine profile of t-lymphocytes of peripheral blood and synovial fluid in rheumatoid arthritis
| Autor: | G B Kolotova, Iu G Lagereva, Ia B Beĭkin |
|---|---|
| Jazyk: | ruština |
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Терапевтический архив, Vol 80, Iss 5, Pp 21-25 (2008) |
| Druh dokumentu: | article |
| ISSN: | 0040-3660 2309-5342 |
| Popis: | Aim. To assess cytokine-producing activity of T-lymphocytes of peripheral blood and synovial fluid in rheumatoid arthritis (RA). Material and methods. The study group consisted of 58 RA female patients aged 28-53 years (mean age 42.1 ± 2.9 years) with at least 5-year history of RA (mean 10.7 ± 4.9 years). The control group consisted of 37 healthy women matched by age. Spontaneous production of IL-2, IL-4, IFNgamma (IFNγ) and TNFalpha (TNFα) by CD3+ and CD3- lymphocytes was estimated after 4 hours of incubation in 37°C with phorbol-12-myristate-13-acetate (PMA, 50 ng/ml, Sigma, France), ionomycin (1 mcg/ml, Sigma, France) and brefeldin A (10 mcg/ml, Sigma, France). Results. In RA there is a decrease in a relative and absolute count of peripheral blood T-lymphocytes which occurs spontaneously and due to nonspecific stimulation of synthesizing IL-4, TNFα and IFNγ. IFNγ-producing lymphocytes (CD3+IFNg+/CD3+IL-4+=4.45 ± 1.56) prevail in the synovial fluid in RA, but count of lymphocyte IFNγ, IL-2 and TNFα+ in the synovial fluid is lower than in peripheral blood. Conclusion. One of the causes of low count of TNFα, IFNγ and IL-4 producing T-lymphocytes in peripheral blood is migration of relevant TC-subpopulations to the affected joints. Migration of TC1-lymphocytes into the synovial fluid does not result in their prevailing count in the synovial fluid vs peripheral blood. |
| Databáze: | Directory of Open Access Journals |
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