Expression of recombinant classical swine fever virus E2 glycoprotein instable High Five cells
| Autor: | EKTA BHARDWAJ, DIPAK DEKA, RAMNEEK VERMA |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Indian Journal of Animal Sciences, Vol 94, Iss 6 (2024) |
| Druh dokumentu: | article |
| ISSN: | 0367-8318 2394-3327 |
| DOI: | 10.56093/ijans.v94i6.145934 |
| Popis: | Classical swine fever is one of the most important viral diseases of domestic pigs and wild boar. It is a notifiable disease to the World Organization for Animal Health. In the present study, codon optimized CSFV E2 gene was cloned into a laboratory-modified insect cell expression vector for producing the recombinant protein in secretory form. Endotoxin-free recombinant plasmid prepared from the positive clone was transfected into the High Five insect cell line and then selected against zeocin antibiotic for the development of a stable cell line. The stable High Five cell line successfully secreted E2 recombinant protein with a molecular weight of ~65 kDa as revealed by SDS-PAGE and Western blot. The recombinant E2 protein showed efficient reactivity with known CSFV anti-serum and monoclonal antibodies (MAbs against E2) in dot blot assay indicating its potential as a diagnostic antigen. |
| Databáze: | Directory of Open Access Journals |
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