Phylogenetic signal at the Cytb-SertRNA-IG1-ND1 mitochondrial region in Anopheles (Kerteszia) neivai Howard, Dyar & Knab, 1913
| Autor: | Andrés López-Rubio, Juan David Suaza, Charles Porter, Sandra Uribe, Gabriel Bedoya, Iván Darío Vélez |
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| Jazyk: | English<br />Spanish; Castilian |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Biomédica: revista del Instituto Nacional de Salud, Vol 37, Iss 0, Pp 143-154 (2017) |
| Druh dokumentu: | article |
| ISSN: | 0120-4157 81471807 |
| DOI: | 10.7705/biomedica.v37i0.3452 |
| Popis: | Introduction: Mitochondrial DNA has proven its utility for the study of insect evolution. Genes such as cytochrome b (Cytb) and the transfer gene for serine (SertRNA) can be used to compare closely related organisms. Objective: The phylogenetic utility of Cytb-SertRNA-IG1-ND1 was tested for polymorphisms, and secondary structure modeling in SertRNA was done to detect possible cryptic species in Anopheles neivai. Materials and methods: Specimens from Colombia, Guatemala, and the type locality in Panamá were collected and sequenced for specimen comparison based on DNA polymorphisms, and secondary structure modeling for the SertRNA gene. Results: Thirty-six sequences for A. neivai and A. pholidotus were obtained. Conclusions: Polymorphic variants were detected in A. neivai for Cytb-SertRNA-IG1- ND1. Despite this variation in A. neivai, cryptic species could not be detected. |
| Databáze: | Directory of Open Access Journals |
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