Immune responses against autologous tumor and human papilloma virus in lymph nodes from patients with penile cancer
| Autor: | Lu Zhang, A. Ali Zirakzadeh, Jesper Rosvall, Mats Hedlund, Ping Sheng Hu, Katrine Riklund, Amir Sherif, Ola Winqvist |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Investigative and Clinical Urology, Vol 62, Iss 1, Pp 39-46 (2021) |
| Druh dokumentu: | article |
| ISSN: | 2466-0493 2466-054X |
| DOI: | 10.4111/icu.20200116 |
| Popis: | Purpose: Nearly half of penile cancers are related to human papillomavirus (HPV) infection. Investigations of tumor- and HPV-specific T cell reactivity in regional lymph nodes (LNs) from patients with penile cancer are warranted. Materials and Methods: In this study, single-cell suspensions from LNs and peripheral blood from 11 patients with penile cancer were stained with antibodies for lymphocyte markers and analyzed by fluorescence-activated cell sorting (FACS). DNA was extracted from the tumor tissue and HPV status was investigated by PCR. Results: T-cell reactivity against autologous tumor-extract and against the HPV-vaccine Gardasil® was tested by flow-cytometric assay of specific cell-mediated immune response in activated whole blood (FASCIA). CD4+/CD8+ ratios were significantly lower in HPV positive LNs (p |
| Databáze: | Directory of Open Access Journals |
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