Autor: |
Natalie Porat-Shliom, Olivia J. Harding, Lenka Malec, Kedar Narayan, Roberto Weigert |
Jazyk: |
angličtina |
Rok vydání: |
2019 |
Předmět: |
|
Zdroj: |
iScience, Vol 11, Iss , Pp 440-449 (2019) |
Druh dokumentu: |
article |
ISSN: |
2589-0042 |
DOI: |
10.1016/j.isci.2018.12.036 |
Popis: |
Summary: Mitochondria are dynamic organelles undergoing fission, fusion, and translocation. These processes have been studied in cultured cells; however, little is known about their regulation in cells within tissues in vivo. We applied four-dimensional intravital microscopy to address this in secretory cells of the salivary gland. We found that mitochondria are organized in two populations: one juxtaposed to the basolateral plasma membrane and the other dispersed in the cytosol. Under basal conditions, central mitochondria exhibit microtubule-dependent motility and low fusion rate, whereas basolateral mitochondria are static and display high fusion rate. Increasing cellular energy demand by β-adrenergic stimulation of regulated exocytosis selectively enhanced motility and fusion of central mitochondria. Inhibition of microtubule polymerization led to inhibition of central mitochondrial motility and fusion and a marked reduction in exocytosis. This study reveals a conserved heterogeneity in mitochondrial positioning and dynamics in exocrine tissues that may have fundamental implications in organ pathophysiology. : Optical Imaging; Cell Biology; Functional Aspects of Cell Biology Subject Areas: Optical Imaging, Cell Biology, Functional Aspects of Cell Biology |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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