Co-Culture of Mouse Blastocysts on A Human Recellularized Endometrial Scaffold: An In Vitro Model for Future Implantation Studies
| Autor: | Elham Sadeghi, Mojtaba Rezazadeh Valojerdi, Mojdeh Salehnia |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Cell Journal, Vol 25, Iss 8, Pp 579-590 (2023) |
| Druh dokumentu: | article |
| ISSN: | 2228-5806 2228-5814 |
| DOI: | 10.22074/cellj.2023.1989926.1236 |
| Popis: | Objective: This study evaluates the interaction of mouse blastocysts as a surrogate embryo on a recellularizedendometrial scaffold by seeding human endometrial mesenchymal cells (hEMCs).Materials and Methods: In this experimental study, prepared decellularized human endometrial tissues werecharacterized by morphological staining, DNA content analysis, and scanning electron microscopic (SEM) analysis.The scaffolds were subsequently recellularized by hEMCs. After seven days of cultivation, the mouse blastocysts wereco-cultured on the recellularized scaffolds for 48 hours. Embryo attachment and implantation within these scaffoldswere evaluated at the morphological, ultrastructural, molecular, and hormonal levels.Results: There was no morphological evidence of cells and nuclei in the decellularized scaffold. DNA contentsignificantly decreased by 89.92% compared to the control group (P |
| Databáze: | Directory of Open Access Journals |
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