| Autor: |
Cristina Cantarutti, Paolo Bertoncin, Alessandra Corazza, Sofia Giorgetti, P. Patrizia Mangione, Vittorio Bellotti, Federico Fogolari, Gennaro Esposito |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Magnetochemistry, Vol 3, Iss 4, p 40 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
2312-7481 |
| DOI: |
10.3390/magnetochemistry3040040 |
| Popis: |
The application of gold nanoparticles (AuNPs) is emerging in many fields, raising the need for a systematic investigation on their safety. In particular, for biomedical purposes, a relevant issue are certainly AuNP interactions with biomolecules, among which proteins are the most abundant ones. Elucidating the effects of those interactions on protein structure and on nanoparticle stability is a major task towards understanding their mechanisms at a molecular level. We investigated the interaction of the 3-mercaptopropionic acid coating of AuNPs (MPA-AuNPs) with β2-microglobulin (β2m), which is a paradigmatic amyloidogenic protein. To this aim, we prepared and characterized MPA-AuNPs with an average diameter of 3.6 nm and we employed NMR spectroscopy and fluorescence spectroscopy to probe protein structure perturbations. We found that β2m interacts with MPA-AuNPs through a highly localized patch maintaining its overall native structure with minor conformational changes. The interaction causes the reversible precipitation of clusters that can be easily re-dispersed through brief sonication. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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