Development of the PCR Assay with Universal Primers for the Detection of Different tcpA Gene Variants

Autor: A. V. Fadeeva, G. A. Eroshenko, G. N. Odinokov, N. A. Sharapova, T. V. Valova, V. V. Kutyrev
Jazyk: ruština
Rok vydání: 2012
Předmět:
Zdroj: Проблемы особо опасных инфекций, Vol 0, Iss 1(111), Pp 102-103 (2012)
Druh dokumentu: article
ISSN: 0370-1069
2658-719X
DOI: 10.21055/0370-1069-2012-1(111)-102-103
Popis: Developed is the PCR assay for the detection of the structural genes of toxin co-regulated adhesion piluses - tcpA of different types. Determined are the universal primers, the usage of which provides for the detection of the stated above genes in V. cholerae of various serogroups. With the help of this PCR assay identified is a new variant of tcpA gene in toxigenic cholera vibrio of non-O1/non-O139 serogroup.
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