| Popis: |
Intestinal epithelial barrier and inflammatory responses are common pathological process in intestinal diseases. Effects of a specific polysaccharide fraction (POPZ) from Portulaca oleracea L. on LPS-induced barrier injury and inflammatory responses were studied in porcine intestinal epithelial monolayer. POPZ was characterized by its molecular constituents with Man, GlcUA, Glc, Gal, Xyl and Fuc (0.247:0.245: 0.225: 0.179: 0.077:0.026, molar ratio), and Mp (41.496 kDa), Mw (51.418 kDa) and Mn (34.455 kDa). POPZ inhibited an increase in FD4 and phenol red permeability, and a decrease in TER and p-EGFR levels, and ZO-1, OCLN, claudin-7 and EGFR expression in LPS-treated porcine intestinal epithelial monolayer, which were counteracted by ZD1839, an EGFR inhibitor. It also ameliorated LPS-induced increase in IL-1β, IL-6, TNF-α, TLR4, NF-κB p-p65 and NF-κB p65 nuclear translocation. Taken together, POPz could relieve inflammatory responses and maintain the intestinal epithelial barrier via inactivating TLR4/NF-κB pathway and activating EGF/EGFR pathway. |
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