| Autor: |
Patricia Goldschmidt Lins, Silvana Marina Piccoli Pugine, Antonio Márcio Scatolini, Mariza Pires de Melo |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Heliyon, Vol 4, Iss 9, Pp e00805- (2018) |
| Druh dokumentu: |
article |
| ISSN: |
2405-8440 |
| DOI: |
10.1016/j.heliyon.2018.e00805 |
| Popis: |
Aims: This study aimed to evaluate in vitro antioxidant capacity of olive leaf extract (OLE), Olea europaea L., and its protective effect on peroxyl radical-induced oxidative damage in human erythrocytes. Main methods: The OLE was evaluated by the following assays: i) total phenolic and flavonoid content; ii) oleuropein content; iii) Ferric reducing antioxidant power (FRAP); iv) antioxidant activity against ABTS·+, DPPH· and reactive oxygen and nitrogen species: superoxide anion (O2·−), hypochlorous acid (HOCl) and nitric oxide (NO·) and v) protective effect on peroxyl radical-induced oxidative damages in human erythrocytes as hemolysis, thiobarbituric acid reactive substances (TBARS) formation and oxyhemoglobin oxidation. Key findings: Total phenolic and flavonoid contents were 131.7 ± 9.4 mg gallic acid equivalents/g dry weight (dw) and 19.4 ± 1.3 mg quercetin equivalents/g dw, respectively. Oleuropein content was 25.5 ± 5.2 mg/g dw. FRAP analysis was 281.8 ± 22.8 mg trolox equivalent/g dw and OLE inhibited ABTS·+ (50% effective concentration (EC50) = 16.1 ± 1.2 μg/mL) and DPPH· (EC50 = 13.8 ± 0.8 μg/mL). The extract demonstrated effective ability to scavenge O2·− (EC50 = 52.6 ± 2.1 μg/mL), NO· (EC50 = 48.4 ± 6.8 μg/mL) and HOCl (EC50 = 714.1 ± 31.4 μg/mL). The extract inhibited peroxyl radical-induced hemolysis (EC50 = 11.5 ± 1.5 μg/mL), TBARS formation (EC50 = 38.0 ± 11.7 μg/mL) and hemoglobin oxidation (EC50 = 186.3 ± 29.7 μg/mL) in erythrocytes. Significance: OLE is an important source of natural antioxidants; it has effective antioxidant activity against different reactive species and protects human erythrocytes against oxidative damage. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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