The Cryopreserved Sperm Traits of Various Ram Breeds: Towards Biodiversity Conservation

Autor: Jakub Vozaf, Andrea Svoradová, Andrej Baláži, Jaromír Vašíček, Lucia Olexiková, Linda Dujíčková, Alexander V. Makarevich, Rastislav Jurčík, Hana Ďúranová, Peter Chrenek
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Animals, Vol 12, Iss 10, p 1311 (2022)
Druh dokumentu: article
ISSN: 12101311
2076-2615
DOI: 10.3390/ani12101311
Popis: The aim of our research was to compare three Slovak sheep breeds in the quality parameters of cryopreserved sperm. The ejaculates of Slovak Dairy (SD), Native Wallachian (NW), and Improved Wallachian (IW) sheep rams (n = 12) were collected by electro-ejaculation. Heterospermic samples were created from suitable ejaculates, separately for each breed (at least 90% of total and 80% of progressive motility). Samples were equilibrated in a Triladyl® diluent and frozen by automated freezing. Sperm samples were subjected to the motility, morphology, (CASA), viability and apoptosis (DRAQ7/Yo-Pro-1), fertilizing capability (penetration/fertilization test (P/F) in vitro) and acrosomal status (transmission electron microscopy) assays before freezing and after thawing. It was found that there were no significant differences (p < 0.05) between the evaluated breeds in motility, viability, apoptosis, morphological properties, and fertilizing ability of cryopreserved sperm. Significant differences occurred in acrosomal status. Our results demonstrate that the use of the selected cryopreservation protocol is suitable for at least three different sheep breeds, which can greatly benefit the biodiversity protection and simplifies the creation of an animal genetic resources gene bank.
Databáze: Directory of Open Access Journals
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