| Autor: |
Álvaro Rodríguez-García, Rosa E. Mares, Patricia L. A. Muñoz, Samuel G. Meléndez-López, Alexei F. Licea-Navarro, Marco A. Ramos |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Methods and Protocols, Vol 1, Iss 4, p 37 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
2409-9279 |
| DOI: |
10.3390/mps1040037 |
| Popis: |
Tuberculosis (TB) remains as a major public health issue in developing countries. Accurate detection is essential for the proper management of patients with active disease. Here, we present a simple DNAzol-LAMP (loop-mediated isothermal amplification) procedure for the detection of Mycobacterium tuberculosis in sputum specimens. Twenty smear-positive sputum samples were analyzed as follows: (i) Genetic material was extracted by a standard DNAzol protocol, and (ii) mycobacterial DNA was detected by a typical TB-specific loop-mediated isothermal amplification method. Results and diagnostic test performance attests to the suitability of the proposed procedure. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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