| Autor: |
NIE Chen, HAN Jingjing, ZHAO Sa, YANG Fan, HE Jingcheng, WANG Peifeng, LI Mingzhe, LIN Chao, ZHANG Hongwei |
| Jazyk: |
čínština |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Zhongguo shipin weisheng zazhi, Vol 34, Iss 4, Pp 755-760 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1004-8456 |
| DOI: |
10.13590/j.cjfh.2022.04.019 |
| Popis: |
ObjectiveTo develop a method for the determination of nosiheptide residue in food animal tissues （porcine muscle， porcine liver， porcine kidney， chicken muscle， chicken liver and chicken kidney） with high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）.MethodsAfter extracted by 0.1% acidic acetonitrile， the nosiheptide extract was cleaned up by Captiva ND cartridge and determined by HPLC-MS/MS in negative electrospray ionization mode. Quantification was performed using corresponding matrix-matched calibration curves.ResultsNosiheptide in corresponding matrix showed good linear relationships （r>0.99） in the calibration range of 2~200 μg/L， and the limit of detection （LOD） and limit of quantification （LOQ） of the method were 2.0 and 7.0 μg/kg， respectively. The recoveries of the method ranged from 76.27% to 92.31% at the spiking level of 3.5， 7.0 and 70 μg/kg with relative standard deviations of 2.15%~8.03%.ConclusionThe proposed method is simple， rapid， accurate as well as reproducible and can be applied to monitor and determine nosiheptide residue in food animal tissues. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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